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Vitrification of Embryos

Vitrification is an exciting new technique which is used to cryopreserve (freeze) supernumerary eggs and embryos.

Vitrification

An IVF cycle will hopefully produce a number of viable embryos. Those that aren’t immediately transferred back to the patient are placed in cryostorage by lowering their temperature to that of liquid nitrogen (-196 ºC). At this temperature, embryos can be stored until they are needed for later transfer. A tiny droplet of cryopreservant fluid containing an embryo holds to its vitrification hook. The problem with freezing any cell in the body is that the fluid inside the cells can form ice crystals that expand and damage the cell membranes. Enormous care must be taken to avoid this. In the past this has meant that eggs and embryos were frozen using a slow programmed decrease of temperature using expensive machinery. With the new vitrification technique using ultrarapid freezing techniques, embryos are cooled at an extreme rate, so that the embryos are flash frozen and preserved intact. Survival rates after vitrification are better than they were with conventional slow freezing. We at Manipal Fertility have a pioneering technology which enables us to achieve post thaw survival rates of 90% for the frozen embryos and a clinical pregnancy rate of 60 – 70% in frozen thawed cycles.

Salient Features:

  • Ultra-rapid cooling of specimens
  • No liquid nitrogen exposure
  • No vapour or straw barriers to heat transfer
  • Visual confirmation of Vitrification
  • Convenient handling of specimens
  • Fast and easy setup and operation times
  • Convenient storing of specimens
  • Ultra-rapid thawing
  • Minimal thawing medium required
  • Specimens easy to locate after thawing

Vitrification Straw

vitrification_straw

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